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Development of functionalized microgels for specific binding of bacterial enterotoxins
 
The aim of the proposal is to develop functionalized microgels that specifically bind bacterial toxins with the goal to be used as a non-antibiotic detoxification therapeutics. Clostridium difficile is an opportunistic path-ogens that colonized the human intestinal tract after disruption of the local microbiota by antibiotic treat-ment. C. difficile produces two toxins, TcdA and TcdB that mediate cytopathic effects after intracellular up-take into host cells resulting in a strong inflammatory response in the colon. TcdA and TcdB are large pro-teins consisting of several functional domains. The receptor binding domains responsible for the binding of the toxin to the host cell membrane contain repeating units with putative binding sites for membrane-associated glycans. Although the natural glycan receptors are still unknown, glycan screens have identified the trisaccharide Galili epitope as a non-human high-affinity ligand for TcdA. During the first funding period, we successfully designed Galili-functionalized microgels that specifically bind TcdA.
 
Principle Investigators:
 
Kühne, Alexander, Dr. rer. nat.,
DWI – Leibniz Institute for Interactive Materials
RWTH Aachen University
+49 241 80-23343
kuehne@dwi.rwth-aachen.de
 
Elling, Lothar, Prof. Dr. rer. nat.
Helmholtz-Institute for Biomedical Engineering
RWTH Aachen University
+49 241 80-28350
 
Trautwein, Christian, Prof. Dr. med.,
Lehrstuhl für Innere Medizin
Uniklinik RWTH Aachen
+49 241 80-80866
 
Sellge, Gernot, Dr. med., Ph.D.
Lehrstuhl für Innere Medizin
Uniklinik RWTH Aachen
+49 241 80-35775